イケダ ツヨシ   Ikeda Tsuyoshi
  池田 剛
   所属   崇城大学  薬学部 薬学科
   崇城大学大学院  薬学研究科 薬学専攻
   職種   教授
言語種別 英語
発行・発表の年月 2008/04
形態種別 原著論文
査読 査読有り
標題 Usefulness of antibodies for evaluating the biological significance of AGEs.
執筆形態 共著
掲載誌名 Annals of the New York Academy of Sciences
掲載区分国外
巻・号・頁 1126,pp.38-41
著者・共著者 Nagai Ryoji, Fujiwara Yukio, Mera Katsumi, Motomura Keita, Iwao Yasunori, Tsurushima Keiichiro, Nagai Mime, Takeo Kazuhiro, Yoshitomi Makiko, Otagiri Masaki, Ikeda Tsuyoshi
概要 We prepared specific monoclonal antibodies against CML, CEL, CMA, and CMC. Our immunochemical study using anti-CMA antibody demonstrated that the CMA content increased in a time-dependent manner when collagen was incubated with glucose, indicating that immunological quantification using the specific antibody is especially useful for measuring an acid-labile AGE structure, such as CMA. Monoclonal antibody is also applied to identify a novel biological marker in pathological lesions. We prepared antibody libraries against proteins modified with aldehydes, such as glyoxal, methylglyoxal, and glycolaldehyde (GA), and one antibody, GA5, which specifically reacts with the GA-modified protein that is recognized in human atherosclerotic lesions. Taken together, these results demonstrate that a specific antibody is a powerful tool for analyzing novel biomarkers, formation pathways, and the efficacy of AGE inhibitors.
DOI 10.1196/annals.1433.001
ISSN 0077-8923
PMID 18079488