イケダ ツヨシ   Ikeda Tsuyoshi
  池田 剛
   所属   崇城大学  薬学部 薬学科
   崇城大学大学院  薬学研究科 薬学専攻
   職種   教授
言語種別 英語
発行・発表の年月 2007/07
形態種別 原著論文
査読 査読有り
標題 alpha-Tomatine, the major saponin in tomato, induces programmed cell death mediated by reactive oxygen species in the fungal pathogen Fusarium oxysporum.
執筆形態 共著
掲載誌名 FEBS letters
掲載区分国外
巻・号・頁 581(17),pp.3217-22
著者・共著者 Ito Shin-Ichi, Ihara Takashi, Tamura Hideyuki, Tanaka Shuhei, Ikeda Tsuyoshi, Kajihara Hiroshi, Dissanayake Chandrika, Abdel-Motaal Fatma F, El-Sayed Magdi A
概要 In the present study, mechanisms involved in alpha-tomatine-induced cell death of fungi were examined using a filamentous pathogenic fungus Fusarium oxysporum. alpha-Tomatine-induced cell death of F. oxysporum (TICDF) occurred only under aerobic conditions and was blocked by the mitochondrial F(0)F(1)-ATPase inhibitor oligomycin, the caspase inhibitor D-VAD-fmk, and protein synthesis inhibitor cycloheximide. Fungal cells exposed to alpha-tomatine showed TUNEL-positive nuclei, depolarization of transmembrane potential of mitochondria, and reactive oxygen species (ROS) accumulation. These results suggest that TICDF occurs through a programmed cell death process in which mitochondria play a pivotal role. Pharmacological studies using inhibitors suggest that alpha-tomatine activates phosphotyrosine kinase and monomeric G-protein signaling pathways leading to Ca(2+) elevation and ROS burst in F. oxysporum cells.
DOI 10.1016/j.febslet.2007.06.010
ISSN 0014-5793
PMID 17585910