イケダ ツヨシ   Ikeda Tsuyoshi
  池田 剛
   所属   崇城大学  薬学部 薬学科
   崇城大学大学院  薬学研究科 薬学専攻
   職種   教授
言語種別 英語
発行・発表の年月 2015/04
形態種別 原著論文
標題 Identification of HSP70-inducing activity in Arnica montana extract and purification and characterization of HSP70-inducers.
執筆形態 共著
掲載誌名 Journal of dermatological science
掲載区分国外
巻・号・頁 78(1),pp.67-75
著者・共著者 Usui Keiko, Ikeda Tsuyoshi, Horibe Yuumi, Nakao Mitsuyoshi, Hoshino Tatsuya, Mizushima Tohru
概要 RESULTS: From the extract, we purified and identified eight sesquiterpene lactones (AM1-8) as HSP70-inducers, among which AM-2 (helenalin 2-methylbutyrate) was selected due to its good HSP70-inducing properties and low cytotoxicity. Treatment of cultured mouse melanoma cells with AM-2 or A. montana extract up-regulated the expression of HSP70 in a dose-dependent manner. This treatment also activated heat shock factor-1, a transcription factor for hsp genes. Furthermore, pre-treatment of cells with AM-2 or A. montana extract decreased melanin production and expression and activity of tyrosinase. CONCLUSION: These results suggest that AM-2 and A. montana extract could be beneficial for use in hypopigmenting cosmetics as a consequence of their stimulatory effects on HSP70 expression.
DOI 10.1016/j.jdermsci.2015.01.014
ISSN 1873-569X
PMID 25724362