イチハラ ヒデアキ   Ichihara Hideaki
  市原 英明
   所属   崇城大学  生物生命学部 応用生命科学科
   崇城大学大学院  工学研究科 応用生命科学専攻(博士前期課程)
   崇城大学大学院  工学研究科 応用生命科学専攻(博士後期課程)
   職種   教授
言語種別 英語
発行・発表の年月 2009/04
形態種別 原著論文
査読 査読有り
標題 Chemotherapy with hybrid liposomes for human breast tumors along with apoptosis in vivo
執筆形態 共著
掲載誌名 International Journal of Pharmaceutics
掲載区分国外
巻・号・頁 372(1-2),pp.162-168
著者・共著者 Shimoda S, Ichihara H, Matsumoto Y, Ueoka R
概要 A clear solution of HL-n composed of 95 mol% dimyristoylphosphatidylcholine (DMPC) and 5 mol% polyoxyethylenedodecyl ether (C12(EO)n, n=21 and 25) having hydrodynamic diameter of 100 nm could be kept over 1 month on the basis of dynamic light scattering measurements was obtained. (1) Increases of fusion and accumulation of HL-n including NBDPC as a fluorescence probe into human breast tumor (MDA-MB-453) cell membranes were observed. (2) Reduction of mitochondrial membrane potential and activation of caspase-8, caspase-9, and caspase-3 were observed, indicating that apoptotic signal by HL-n should pass through mitochondria and these caspases. (3) Remarkable reduction of tumor volume in a xenograft mice model intravenously treated with HL-n without drugs after the subcutaneously inoculation of MDA-MB-453 cells was verified in vivo. Induction of apoptosis in tumor of xenograft mice treated with HL-n was observed in micrographs on the basis of TUNEL method.
DOI 10.1016/j.ijpharm.2009.01.011