Faculty Information - Ookuri Takatoshi

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Book and thesis

Academic conference presentation

Academic background

Business career

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Present specialized field

Department laboratory expense researcher number

（Last updated : 2022-10-24 17:54:24）

Ookuri Takatoshi
Department SOJO UNIVERSITY Department of Pharmaceutical Sciences, FACULTY OF PHARMACEUTICAL SCIENCES

Position

■ Book and thesis

1.	2022/06	Article	Effect of an intermolecular disulfide bond introduced into the first loop of CH1 domain of Adalimumab Fab on thermal stability and antigen-binding activity. The Journal of Biochemistry 172(1),pp.49-56 (Collaboration)
2.	2021/10	Article	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies. The Journal of Biochemistry 170(2),pp.289-297 (Collaboration)
3.	2021/06	Article	A comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab. Scientific reports 11(1),pp.12937 (Collaboration)
4.	2021/06	Article	Abolition of aggregation of CH 2 domain of human IgG1 when combining glycosylation and protein stabilization Biochem Biophys Res Commun. 18(558),pp.114-119 (Collaboration)
5.	2021/04	Article	Glycosylation decreases aggregation and immunogenicity of adalimumab Fab secreted from Pichia pastoris. The Journal of Biochemistry 29(169),pp.435-443 (Collaboration)
6.	2020/03	Article	C-Terminal Cysteine PEGylation of Adalimumab Fab with an Engineered Interchain SS Bond. Biol Pharm Bull. 1(43),pp.418-423 (Collaboration)
7.	2020/01	Article	Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader: Structural basis implying a distortion-accumulation mechanism for the DnaB ring opening caused by DnaC binding The Journal of Biochemistry 167(1),pp.1-14 (Collaboration)
8.	2019/12	Article	A method to induce hen egg lysozyme-specific humoral immune tolerance in mice by pre-exposition with the protein's oligomers. Biochemistry and Biophysics Reports 14(20),pp.100679 (Collaboration)
9.	2018/09	Article	Introduction of a glycosylation site in the constant region decreases the aggregation of adalimumab Fab. Biochem Biophys Res Commun. 5(503),pp.752-756 (Collaboration)
10.	2018/01	Article	A novel engineered interchain disulfide bond in the constant region enhances the thermostability of adalimumab Fab. Biochem Biophys Res Commun. 495(1),pp.7-11 (Collaboration)
11.	2016/10	Article	Relationship between the magnitude of IgE production in mice and conformational stability of the house dust mite allergen, Der p 2. 1860(10),pp.2279-2284 (Collaboration)
12.	2015/05	Article	Role of the osmolyte taurine on the folding of a model protein, hen egg white lysozyme, under a crowding condition. Amino Acids 47(5),pp.909-915 (Collaboration)
13.	2014/12	Article	Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A2 isozyme genes. Toxicon 15(92),pp.133-139 (Collaboration)
14.	2013/10	Article	Characterization of deamidation at Asn138 in L-chain of recombinant humanized Fab expressed from Pichia pastoris. The Journal of Biochemistry 154(4),pp.333-340 (Collaboration)
15.	2013/08	Article	Domain separation and characterization of PriC, a replication restart primosome factor in Escherichia coli. Genes Cells. 18(9),pp.723-732 (Collaboration)
16.	2013/04	Article	Mechanism for retardation of amyloid fibril formation by sugars in Vλ6 protein. Protein Sci. 22(4),pp.467-474 (Collaboration)
17.	2013/03	Article	Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor. The Journal of Biochemistry 153(3),pp.275-282 (Collaboration)
18.	2011/12	Article	Aggregates with lysozyme and ovalbumin show features of amyloid-like fibrils. Biochem Cell Biol. 89(6),pp.533-544 (Collaboration)
19.	2010	Article	A protein's conformational stability is an immunologically dominant factor: evidence that free-energy barriers for protein unfolding limit the immunogenicity of foreign proteins. Journal of immunology 185(7),pp.4199-4205 (Collaboration)
20.	2010	Article	Effects of His mutations on the fibrillation of amyloidogenic Vlambda6 protein Wil under acidic and physiological conditions . Biochem. Biophys. Res Commun. 391(1),pp.615-620 (Collaboration)
21.	2010	Article	Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form. Arch. Biochem. Biophys. 494(2),pp.145-150 (Collaboration)
22.	2010	Article	Inhibitory effects of SSRIs on IFN-γ induced microglial activation through the regulation of intracellular calcium. Prog. Neuropsycho. Biol .Psychiatry. 494(2),pp.1306-1316 (Collaboration)
23.	2009	Article	Residual structures in the acid-unfolded states of Vlambda6 proteins affect amyloid fibrillation. J. Mol. Biol. 392(4),pp.1033-1043 (Collaboration)
24.	2008	Article	Crystal structures of K33 mutant hen lysozymes with enhanced activities J. Biochem. 144(5),pp.619-623 (Collaboration)
25.	2007	Article	Analyses of native disulfide bond formations on the early stage of the folding process in mutant lysozymes where the long range interactions in the denatured state were modulated. Biosci. Biotechnol. Biochem. 71(8),pp.2072-2074 (Collaboration)
26.	2007	Article	Analysis of internal motions of interleukin-13 variant associated with severe bronchial asthma using ¹⁵N NMR relaxation measurements. Biochem. Biophys. Res Commun. 358(1),pp.292-297 (Collaboration)
27.	2007	Article	Expression of human IL-13 receptor alpha2 extracellular domain in Pichia pastoris. Protein expression and purification. 56(1),pp.48-53 (Collaboration)
28.	2007	Article	Extremely thermophilic translation system in the common ancestor Commonote: ancestral mutants of Glycyl-tRNA synthetase from the extreme thermophile Thermus themophilus. J. Mol. Biol. 369(4),pp.1060-1069 (Collaboration)
29.	2007	Article	Stable supply of large amounts of human Fab from the inclusion bodies in E. coli. The Journal of Biochemistry 141(5),pp.699-707 (Collaboration)
30.	2007	Article	The effects of mutations at position 253 on the thermostability of the Bacillus subtilis3-isopropylmalate dehydrogenase subunit interface. The Journal of Biochemistry 141(6),pp.791-797 (Collaboration)
31.	2007	Article	A particular hydrophobic cluster in the residual structure of reduced lysozyme drastically affects the amyloid fibrils formation. Biochem Biophys Res Commun. 356(3),pp.769-772 (Collaboration)
32.	2006	Article	Amyloid formation in denatured single-mutant lysozymes where residual structures are modulated. Protein Sci. 15(10),pp.2448-2452 (Collaboration)
33.	2006	Article	Analysis of internal motions of RNase TI complexed with a productive substrate involving¹⁵N NMR relaxation measurements. The Journal of Biochemistry 140(1),pp.43-48 (Collaboration)
34.	2006	Article	Designing thermostable proteins: Ancestral mutants of 3-isopropylmalate dehydrogenase designed by using a phylogenetic tree. J. Mol. Biol. 355(4),pp.664-674 (Collaboration)
35.	2006	Book	Protein Folding: New Research. pp.173-194 (Collaboration)
36.	2005/12	Article	Effect of W62G mutation of hen lysozyme on the folding in vivo. Biochem Biophys Res Commun. 338(2),pp.820-824 (Collaboration)
37.	2005/06	Article	Characterization of refolded hen lysozyme variant lacking two outside disulfide bonds. Biosci Biotechnol Biochem. 69(6),pp.1206-1208 (Collaboration)
38.	2005/03	Article	Effect of the structure of the denatured state of lysozyme on the aggregation reaction at the early stages of folding from the reduced form. J Mol Biol. 347(1),pp.159-168 (Collaboration)
39.	2005/02	Article	Thermostability of ancestral mutants of Caldococcus noboribetus isocitrate dehydrogenase. FEMS Microbiol Lett. 243(2),pp.393-398 (Collaboration)
40.	2003/08	Article	Increased thermal stability against irreversible inactivation of 3-isopropylmalate dehydrogenase induced by decreased van der Waals volume at the subunit interface. Protein Eng. 16(8),pp.615-621 (Collaboration)
41.	2002/07	Article	A metal binding in the polypeptide chain improves the folding efficiency of a denatured and reduced protein. Biopolymers. 64(2),pp.106-114 (Collaboration)
42.	2001/11	Article	Evidence for an initiation site for hen lysozyme folding from the reduced form using its dissected peptide fragments. Protein Eng. 14(11),pp.829-833 (Collaboration)
43.	1996/11	Article	Identification of the peptide region that folds native conformation in the early stage of the renaturation of reduced lysozyme. Biochem Biophys Res Commun. 228(1),pp.203-208 (Collaboration)
44.	1995/12	Article	Kinetically trapped structure in the renaturation of reduced oxindolealanine 62 lysozyme. Biochemistry. 34(49),pp.16178-16185 (Collaboration)
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■ Academic conference presentation

1.	2019/09/20	Introduction of a glycosylation site in the constant region of human Fab and effect of the effect of glycosylation on the properties of the Fab
2.	2019/06/25	Preparation of C-terminal specific PEG modified Fab with intermolecular disulfide bond

■ Academic background

1.	1990/04～1994/03	Faculty of Pharmaceutical Science, Fukuoka University, Graduated,
2.	1994/04～1996/03	〔Master Course〕, Graduate School, Division of Pharmaceutical Sciences, Kyushu University, Completed,
3.	1996/04～1999/03	〔Doctorial Course〕, Graduate School, Division of Pharmaceutical Sciences, Kyushu University, Completed,

■ Business career

1.	2002/04～2008/03	Research Assistant
2.	2008/04～2009/09	Assistant Professor
3.	2009/10～2020/03	SOJO UNIVERSITY FACULTY OF PHARMACEUTICAL SCIENCES Department of Pharmaceutical Sciences
4.	2012/04～	/(DC)Division of Pharmaceutical Sciences

■ Home Page

http://www.ph.sojo-u.ac.jp/~ohkuri/index.html

■ E-Mail Address

■ Present specialized field

Pharmaceutical analytical chemistry and physicochemistry, Immunology, Pharmaceutical analytical chemistry and physicochemistry

■ Department laboratory expense researcher number

70346807

■