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Ikeda Tsuyoshi
Department SOJO UNIVERSITY Department of Pharmaceutical Sciences, FACULTY OF PHARMACEUTICAL SCIENCES /(DC)Division of Pharmaceutical Sciences, Position |
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| Language | English |
| Publication Date | 2008/04 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Usefulness of antibodies for evaluating the biological significance of AGEs. |
| Contribution Type | |
| Journal | Annals of the New York Academy of Sciences |
| Journal Type | Another Country |
| Volume, Issue, Page | 1126,pp.38-41 |
| Author and coauthor | Nagai Ryoji, Fujiwara Yukio, Mera Katsumi, Motomura Keita, Iwao Yasunori, Tsurushima Keiichiro, Nagai Mime, Takeo Kazuhiro, Yoshitomi Makiko, Otagiri Masaki, Ikeda Tsuyoshi |
| Details | We prepared specific monoclonal antibodies against CML, CEL, CMA, and CMC. Our immunochemical study using anti-CMA antibody demonstrated that the CMA content increased in a time-dependent manner when collagen was incubated with glucose, indicating that immunological quantification using the specific antibody is especially useful for measuring an acid-labile AGE structure, such as CMA. Monoclonal antibody is also applied to identify a novel biological marker in pathological lesions. We prepared antibody libraries against proteins modified with aldehydes, such as glyoxal, methylglyoxal, and glycolaldehyde (GA), and one antibody, GA5, which specifically reacts with the GA-modified protein that is recognized in human atherosclerotic lesions. Taken together, these results demonstrate that a specific antibody is a powerful tool for analyzing novel biomarkers, formation pathways, and the efficacy of AGE inhibitors. |
| DOI | 10.1196/annals.1433.001 |
| ISSN | 0077-8923 |
| PMID | 18079488 |