Ikeda Tsuyoshi
   Department   SOJO UNIVERSITY  Department of Pharmaceutical Sciences, FACULTY OF PHARMACEUTICAL SCIENCES
     /(DC)Division of Pharmaceutical Sciences,
Language English
Publication Date 2008/04
Peer Review Peer reviewed
Title Usefulness of antibodies for evaluating the biological significance of AGEs.
Contribution Type
Journal Annals of the New York Academy of Sciences
Journal TypeAnother Country
Volume, Issue, Page 1126,pp.38-41
Author and coauthor Nagai Ryoji, Fujiwara Yukio, Mera Katsumi, Motomura Keita, Iwao Yasunori, Tsurushima Keiichiro, Nagai Mime, Takeo Kazuhiro, Yoshitomi Makiko, Otagiri Masaki, Ikeda Tsuyoshi
Details We prepared specific monoclonal antibodies against CML, CEL, CMA, and CMC. Our immunochemical study using anti-CMA antibody demonstrated that the CMA content increased in a time-dependent manner when collagen was incubated with glucose, indicating that immunological quantification using the specific antibody is especially useful for measuring an acid-labile AGE structure, such as CMA. Monoclonal antibody is also applied to identify a novel biological marker in pathological lesions. We prepared antibody libraries against proteins modified with aldehydes, such as glyoxal, methylglyoxal, and glycolaldehyde (GA), and one antibody, GA5, which specifically reacts with the GA-modified protein that is recognized in human atherosclerotic lesions. Taken together, these results demonstrate that a specific antibody is a powerful tool for analyzing novel biomarkers, formation pathways, and the efficacy of AGE inhibitors.
DOI 10.1196/annals.1433.001
ISSN 0077-8923
PMID 18079488