イチハラ ヒデアキ   Ichihara Hideaki
  市原 英明
   所属   崇城大学  生物生命学部 応用生命科学科
   崇城大学大学院  工学研究科 応用生命科学専攻(博士前期課程)
   崇城大学大学院  工学研究科 応用生命科学専攻(博士後期課程)
   職種   教授
言語種別 英語
発行・発表の年月 2010/02
形態種別 原著論文
査読 査読有り
標題 Inhibitory effects of cationic hybrid liposomes on the growth of human renal cell carcinoma
執筆形態 共著
掲載誌名 Anticancer Research
掲載区分国外
巻・号・頁 30(2),pp.327-338
著者・共著者 Umebayashi M, Makizono T, Ichihara H, Matsumoto Y, Ueoka R
概要 Cationic hybrid liposomes (HL) can be prepared by simply ultrasonicating a mixture of L-alpha-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene (21)dodecyl ether (C12(EO)21) and O,O'-ditetradecanoyl-N-(alpha-trimethylammonioacetyl) diethanolamine chloride (2C14ECl) in a buffer solution. The inhibitory effects of cationic HL containing 2C14ECl on the growth of OS-RC-2 human renal carcinoma cells in vitro and in vivo were examined. The 50% inhibitory concentration of cationic HL was remarkably reduced compared with that of DMPC liposomes. Induction of apoptosis by cationic HL in OS-RC-2 cells was verified on the basis of flow cytometric analysis and fluorescence microscopy in vitro. In addition, the effects on survival of cationic HL along with apoptosis in vivo were obtained using a mouse model of renal carcinoma.