イチハラ ヒデアキ   Ichihara Hideaki
  市原 英明
   所属   崇城大学  生物生命学部 応用生命科学科
   崇城大学大学院  工学研究科 応用生命科学専攻(博士前期課程)
   崇城大学大学院  工学研究科 応用生命科学専攻(博士後期課程)
   職種   教授
言語種別 英語
発行・発表の年月 2016/01
形態種別 原著論文
査読 査読有り
標題 Therapeutic effects of trehalose liposomes against lymphoblastic leukemia leading to apoptosis in vitro and in vivo
執筆形態 共著
掲載誌名 Bioorganic & Medicinal Chemistry Letters
掲載区分国外
出版社・発行元 Elsevier
巻・号・頁 26(2),pp.301-305
著者・共著者 Y. Matsumoto, K. Kuwabara, H. Ichihara, M. Kuwano
概要 Inhibitory effects of trehalose liposomes (DMTre) composed of 30mol% l-α-dimyristoylphosphatidylcholine (DMPC) and 70mol% trehalose surfactants on the growth of lymphoblastic leukemia (MOLT-4) cells in vitro and therapeutic effects of DMTre for xenograft mice model of carcinoma in vivo were examined. DMTre inhibited the growth of MOLT-4 cells in a dose-dependent manner due to apoptosis. The activation of caspase-3, -8, and 9 was obtained for MOLT-4 cells after the treatment with DMTre. The clustering of lipid rafts in plasma membranes of MOLT-4 cells was examined with a marker Cholera toxin subunit B conjugates Alexa Fluor (CTB), which binds to the pentasaccharide chains of ganglioside GM1 on the cellular surfaces. The clustering of lipid rafts in plasma membranes of MOLT-4 cells was observed after the treatment with DMTre. Therapeutic effects of DMTre were obtained for xenograft mice model of carcinoma in vivo.
DOI 10.1016/j.bmcl.2015.12.025