コミズ　ユウジ   Komizu Yuuji   古水　雄志    所属   崇城大学  生物生命学部 生物生命学科    崇城大学大学院  工学研究科 応用生命科学専攻（博士前期課程）    崇城大学  生物生命学部 応用生命科学科    崇城大学大学院  工学研究科 応用生命科学専攻（博士後期課程）    職種   准教授
言語種別	英語
発行・発表の年月	2014/01
形態種別	原著論文
査読	査読あり
標題	Cell cycle arrest by hybrid liposomes for human lung carcinoma cells
執筆形態	共著
掲載誌名	J. Carcinog. Mutagen.
巻・号・頁	5(1),pp.1000157-1-6
著者・共著者	Komizu Y, Yukihara M, Matsumoto Y, Ueoka R
概要	Hybrid liposomes (HL), composed of L-α-dimyristoylphosphatidylcholine and polyoxyethylene(23) dodecyl ethers, were simply prepared by the sonication method. In this study, we investigated the effects of HL on cell cycle and apoptosis in human non-small cell lung cancer cells. Induction of cell cycle arrest at the G0/G1 phase and apoptosis by HL were observed in human non-small cell lung cancer cells (A549, H460 and H23). HL treatment also resulted in the induction of cyclin-dependent kinases inhibitor p21WAF1/CIP1 and p27KIP1 and a decrease in the protein expressions of cyclins D1 and E. It is noteworthy that the treatment of A549 cells with HL inhibited phosphorylation of Akt in a time- and dose-dependent manner. Furthermore, HL treatment inhibited the filopodia formation in A549 cells. These results suggest that HL-induced cell cycle arrest at the G0/G1 phase could be associated by the up-regulation of cdk inhibitor p21 and p27 through blocking Akt signaling
DOI	doi: 10.4172/2157-2518.1000157