コミズ　ユウジ   Komizu Yuuji   古水　雄志    所属   崇城大学  生物生命学部 生物生命学科    崇城大学大学院  工学研究科 応用生命科学専攻（博士前期課程）    崇城大学  生物生命学部 応用生命科学科    崇城大学大学院  工学研究科 応用生命科学専攻（博士後期課程）    職種   准教授
言語種別	英語
発行・発表の年月	2012/07
形態種別	原著論文
査読	査読あり
標題	Residual powders from Shochu distillation remnants induce apoptosis in human hepatoma cells via the caspase-independent pathway
執筆形態	共著
掲載誌名	J. Biosci. Bioeng.
巻・号・頁	114(1),pp.104-109
著者・共著者	Ohgidani M, Komizu Y, Goto K, Ueoka R
概要	Shochu distillation remnants (SDR) are by-products in the manufacturing process of the Japanese liquor Shochu and include various useful organic compounds derived from the fermentation of grains. We have obtained valuable powder (PSDR) from freeze-dried SDR by the treatment with ethanol. In this study, we examined the anticancer effects of barley-, rice-, and sweet potato-PSDR against human hepatocellular carcinoma (HCC) in vitro. All PSDR inhibited the growth of both these HCC cells through the induction of apoptosis. Especially, barley-PSDR was the most effective for the growth inhibition and apoptosis induction of HCC cells of all PSDR. Furthermore, barley-PSDR induced the nuclear translocation of apoptosis-inducing factor (AIF) from mitochondria, while it did not significantly affect the activities of caspase-3, -8, and -9. The results suggested that barley-PSDR induced apoptosis against HCC cells via the caspase-independent mitochondrial pathway.
DOI	http://dx.doi.org/10.1016/j.jbiosc.2012.02.026