Faculty Information - Ishida Seiichi

Ishida Seiichi

Department / Course FACULTY OF BIOTECHNOOLOGY AND LIFE SCIENCE Department of Biotechnology and Life Sciences

Job

Book and thesis
1.	2025/12	Papers	Double-Layered Microphysiological Systems Improve Cell Adhesion, Viability, and Mitochondrial Activity in Human Hepatocyte Culture 30 (2),pp.70-76 (Co-authored)
2.	2025/11	Papers	Stable and functional human intestinal epithelium derived from induced pluripotent stem cells Drug Metabolism and Disposition,pp.100170 (Co-authored)
3.	2025/09	Papers	Considerations for developing CYP induction assays in hepatocytes: Insights from a multilaboratory study European Journal of Cell Biology 104 (3),pp.151497 (Co-authored)
4.	2025/09	Papers	Enhanced Functional Expression of Salt Export Pump (BSEP) by Three-dimensional Culture of Human Hepatic Cell Line AATEX 30 (1),pp.1-8 (Co-authored)
5.	2025/06	Papers	Approaches to Improving Human Extrapolation of Drug Evaluation Methods 薬学雑誌 145 (6),pp.489-490 (Co-authored)
6.	2025/02	Papers	Global expansion of microphysiological systems (MPS) and Japan's initiatives: Innovation in pharmaceutical development and path to regulatory acceptance. Drug metabolism and pharmacokinetics 60,pp.101047 (Co-authored)
7.	2025/02	Papers	Biology-inspired dynamic microphysiological system approaches to revolutionize basic research, healthcare and animal welfare. ALTEX 42 (2),pp.204-223 (Co-authored)
8.	2024/12	Papers	Enhanced Functionality of Human Hepatocytes using the Oxygen Permeable Culture Plate for Optimized Oxygen Supply in Co-culture Systems with Culture Inserts AATEX 29 (2),pp.64-75 (Co-authored)
9.	2024/04	Papers	Selective elimination of tumorigenic cells from mixed culture of normal and tumorigenic cells using hybrid liposomes aimed at realizing of cell therapy. Cytotechnology 76 (2),pp.247-258 (Co-authored)
10.	2024	Papers	Novel Screening System for Biliary Excretion of Drugs Using Human Cholangiocyte Organoid Monolayers with Directional Drug Transport. Biological & pharmaceutical bulletin 47 (2),pp.427-433 (Co-authored)
11.	2023/06	Papers	Label-Free Evaluation of Maturation and Hepatotoxicity of Human iPSC-Derived Hepatocytes Using Hyperspectral Raman Imaging. Analytical chemistry 95 (24),pp.9252-9262 (Co-authored)
12.	2023/03	Papers	Usefulness of a humanized tricellular static transwell blood-brain barrier model as a microphysiological system for drug development applications. - A case study based on the benchmark evaluations of blood-brain barrier microphysiological system. Regenerative therapy 22,pp.192-202 (Co-authored)
13.	2023	Papers	[Accelerate New Drug Development by Imitating the Biological Environment in Micro-space-The Cutting Edge of MPS from Japan]. Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 143 (1),pp.37-38 (Co-authored)
14.	2023	Papers	Construction of extended and functional bile canaliculi using long-term sandwich-cultured cryopreserved human hepatocytes and the application of hepatocytes for predicting the biliary excretion of pharmaceutical and food-related compounds. The Journal of toxicological sciences 48 (5),pp.251-261 (Co-authored)
15.	2022/09/07	Papers	Construction of a culture protocol for functional bile canaliculi formation to apply human iPS cell-derived hepatocytes for cholestasis evaluation. Scientific reports 12 (1),pp.15192-15192 (Co-authored)
16.	2022/08	Papers	Label-free chemical imaging of cytochrome P450 activity by Raman microscopy. Communications biology 5 (1),pp.778 (Co-authored)
17.	2022/07	Papers	Transcriptional and Epigenetic Consequences of DMSO Treatment on HepaRG Cells. Cells 11 (15) (Co-authored)
18.	2022	Papers	A physiologically based kinetic modeling of ethyl tert-butyl ether in humans-An illustrative application of quantitative structure-property relationship and Monte Carlo simulation. The Journal of toxicological sciences 47 (2),pp.77-87 (Co-authored)
19.	2022	Papers	Directional Drug Transport through Membrane-Supported Monolayers of Human Liver-Derived Cell Lines. Biological & pharmaceutical bulletin 45 (1),pp.150-153 (Co-authored)
20.	2022	Papers	Evaluation of Liver Fibrosis Induction and Inhibition using Human Cultured Hepatic Stellate Cell Spheroid Alternatives to Animal Testing and Experimentation 27 (2),pp.41-52 (Co-authored)
21.	2022	Papers	Human Liver-derived Cell Lines on the Membrane Have a Direction for Drug Transport 45 (1),pp.150-153 (Co-authored)
22.	2021/08/31	Papers	Evaluation for Inhibitory Effects of Hybrid Liposomes on the Growth of Breast Cancer Stem Cells using Soft Agar Colony Formation Assay Alternatives to Animal Testing and Experimentation 26 (1),pp.1-8 (Co-authored)
23.	2021	Papers	Application of Microphysiological Systems (MPS) to Drug Safety and Toxicity Testing and Challenges to Its Industrial and Regulatory Implementation レギュラトリーサイエンス学会誌,pp.43-51 (Sole-authored)
24.	2020/12/01	Papers	Recent Progress in Prediction Systems for Drug-induced Liver Injury Using in vitro Cell Culture. Drug metabolism letters (Co-authored)
25.	2020/10/15	Papers	Immortalization of human hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing. Scientific reports 10 (1),pp.17503-17503 (Co-authored)
26.	2020/04	Papers	Requirements of Microphysiological Systems for industrial acceptance,pp.187-190 (Sole-authored)
27.	2020	Papers	A Hepatocelluar Co-Culture System for the Investigation of Cellular-Based Mechanisms of Troglitazone-Induced Liver Injury. Japanese Journal of Gastroenterology and Hepatology 4 (4),pp.1-5 (Co-authored)
28.	2020	Papers	Culture density contributes to hepatic functions of fresh human hepatocytes isolated from chimeric mice with humanized livers: Novel, long-term, functional two-dimensional in vitro tool for developing new drugs. PloS one 15 (9),pp.e0237809 (Co-authored)
29.	2020	Papers	Development of a New Culture Vessel using Hydrogen-rich Gelatin for Evaluation of Hydrogen Gas Effect on Cellular Toxicity Alternatives to Animal Testing and Experimentation 25 (2),pp.41-47 (Co-authored)
30.	2020	Papers	Usefulness and limitations of mRNA measurement in HepaRG cells for evaluation of cytochrome P450 induction Fundamental Toxicological Sciences 7 (1),pp.9-14 (Co-authored)
31.	2019/09	Books	Requirements for designing organ-on-a-chip platforms to model the pathogenesis of liver disease. Organ-on-a-chip (Sole-authored)
32.	2019/07/02	Papers	Collagen vitrigel promotes hepatocytic differentiation of induced pluripotent stem cells into functional hepatocyte-like cells. Biology open 8 (7) (Co-authored)
33.	2019/01/29	Papers	Inhibitory effect of hybrid liposomes on the growth of liver cancer stem cells. Biochemical and biophysical research communications 509 (1),pp.268-274 (Co-authored)
34.	2019	Papers	[Microphysiological system as a promising technology for drug assay]. Nihon yakurigaku zasshi. Folia pharmacologica Japonica 154 (6),pp.345-351 (Co-authored)
35.	2019	Papers	Efforts for the development of Pharmacokinetic Tests aimed at Alternatives to Animal Experiments Considering OECD's movements. 薬物動態学会ニューズレター (Sole-authored)
36.	2019	Papers	Generation of Human iPSC-Derived Intestinal Epithelial Cell Monolayers by CDX2 Transduction. Cellular and molecular gastroenterology and hepatology 8 (3),pp.513-526 (Co-authored)
37.	2019	Papers	Prediction of Human Hepatic Clearance for Cytochrome P450 Substrates via a New Culture Method Using the Collagen Vitrigel Membrane Chamber and Fresh Hepatocytes Isolated from Liver Humanized Mice. Biological & pharmaceutical bulletin 42 (3),pp.348-353 (Co-authored)
38.	2018/02	Papers	Organs-on-a-chip: Current applications and consideration points for in vitro ADME-Tox studies. Drug metabolism and pharmacokinetics 33 (1),pp.49-54 (Sole-authored)
39.	2018	Papers	A long-term culture system based on a collagen vitrigel membrane chamber that supports liver-specific functions of hepatocytes isolated from mice with humanized livers. The Journal of toxicological sciences 43 (8),pp.521-529 (Co-authored)
40.	2018	Papers	Deactivation of Hepatic Stellate Cells by Culturing on VECELL Inserts Alternatives to Animal Testing and Experimentation 23 (2),pp.53-62 (Co-authored)
41.	2017/08	Papers	【医薬品・化成品開発のための培養工学】創薬プロセスに応用されるインビトロ細胞アッセイ法生物工学会誌,pp.451-454 (Sole-authored)
42.	2017	Papers	A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform Lab on a Chip 18 (1),pp.115-125 (Co-authored)
43.	2017	Papers	Maintenance of viability and functional expression of cryopreserved human hepatocytes using silicate fiber-based three-dimensional scaffold Alternatives to Animal Testing and Experimentation 22 (1),pp.101-106 (Co-authored)
44.	2016/11	Papers	Increase of β2-integrin on adhesion of THP-1 cells to collagen vitrigel membrane. Bioscience, biotechnology, and biochemistry 80 (11),pp.2271-2276 (Co-authored)
45.	2016/09/15	Papers	Design, synthesis, and evaluation of Trolox-conjugated amyloid-β C-terminal peptides for therapeutic intervention in an in vitro model of Alzheimer's disease. Bioorganic & medicinal chemistry 24 (18),pp.4138-4143 (Co-authored)
46.	2016/05/31	Papers	Late stage definitive endodermal differentiation can be defined by Daf1 expression. BMC developmental biology 16 (1),pp.19-19 (Co-authored)
47.	2016/02	Papers	Upregulations of metallothionein gene expressions and tolerance to heavy metal toxicity by three dimensional cultivation of HepG2 cells on VECELL 3-D inserts. The Journal of toxicological sciences 41 (1),pp.147-153 (Co-authored)
48.	2016	Papers	Maintenance of Hepatic Progenitor-like Characteristics of HepaRG Cells by Cultivation on VECELL Inserts Alternatives to Animal Testing and Experimentation 21 (2),pp.71-79 (Co-authored)
49.	2016	Papers	Suspension Culture Improves Cryopreservation- induced Damage of Liver-derived Cells Alternatives to Animal Testing and Experimentation 21 (2),pp.63-70 (Co-authored)
50.	2015/04	Papers	Opportunities to integrate new approaches in genetic toxicology: an ILSI-HESI workshop report. Environmental and molecular mutagenesis 56 (3),pp.277-285 (Co-authored)
51.	2014/12	Papers	Inflammatory cytokines promote the retrodifferentiation of tumor-derived hepatocyte-like cells to progenitor cells. Hepatology (Baltimore, Md.) 60 (6),pp.2077-2090 (Co-authored)
52.	2014	Papers	Comparative metabolome analysis of cultured fetal and adult hepatocytes in humans. The Journal of toxicological sciences 39 (5),pp.717-723 (Co-authored)
53.	2009/12	Papers	Novel biphenylcarboxylic acid peroxisome proliferator-activated receptor (PPAR) delta selective antagonists. Bioorganic & medicinal chemistry letters 19 (23),pp.6595-6599 (Co-authored)
54.	2009/11	Papers	Drug-metabolising enzymes are down-regulated by hypoxia in differentiated human hepatoma HepaRG cells: HIF-1alpha involvement in CYP3A4 repression. European journal of cancer (Oxford, England : 1990) 45 (16),pp.2882-2892 (Co-authored)
55.	2009/01	Papers	Global gene expression changes including drug metabolism and disposition induced by three-dimensional culture of HepG2 cells-Involvement of microtubules BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 378 (3),pp.558-562 (Co-authored)
56.	2008/09	Papers	Decreased expression of cytochromes P450 1A2, 2E1, and 3A4 and drug transporters Na+-taurocholate-cotransporting polypeptide, organic cation transporter 1, and organic anion-transporting peptide-C correlates with the progression of liver fibrosis in chronic hepatitis C patients. Drug metabolism and disposition: the biological fate of chemicals 36 (9),pp.1786-1793 (Co-authored)
57.	2007/09	Papers	Maternal vitamin A alters gene profiles and structural maturation of the rat ductus arteriosus. Physiological genomics 31 (1),pp.139-157 (Co-authored)
58.	2007	Books	How DNA microarray technology contributes to the retinoid evaluations (Co-authored)
59.	2006/03	Papers	Gene expression property of high-density three-dimensional tissue of HepG2 cells formed in radial-flow bioreactor. Journal of bioscience and bioengineering 101 (3),pp.243-250 (Co-authored)
60.	2006/03	Papers	Identification of novel E2F1 target genes regulated in cell cycle-dependent and independent manners. Oncogene 25 (12),pp.1786-1798 (Co-authored)
61.	2005/11	Papers	Polymorphic tandem repeat sequences of the thymidylate synthase gene correlates with cellular-based sensitivity to fluoropyrimidine antitumor agents. Cancer chemotherapy and pharmacology 56 (5),pp.465-472 (Co-authored)
62.	2005/09	Papers	Thyroid hormone targets matrix Gla protein gene associated with vascular smooth muscle calcification. Circulation research 97 (6),pp.550-557 (Co-authored)
63.	2005/06	Papers	New approaches to mechanism analysis for drug discovery using DNA microarray data combined with KeyMolnet. Current drug discovery technologies 2 (2),pp.89-98 (Co-authored)
64.	2005/05	Papers	Gene expression profiling of dexamethasone-treated RBL-2H3 cells: induction of anti-inflammatory molecules. Immunology letters 98 (2),pp.272-279 (Co-authored)
65.	2005/04/28	Papers	FUNCTIONAL ANALYSIS OF SIX HUMAN ARYL HYDROCARBON RECEPTOR VARIANTS IN A JAPANESE POPULATION Drug Metabolism and Disposition 33 (8),pp.1254-1260 (Co-authored)
66.	2005/03	Papers	Three-dimensional high-density culture of HepG2 cells in a 5-ml radial-flow bioreactor for construction of artificial liver. Journal of bioscience and bioengineering 99 (3),pp.237-244 (Co-authored)
67.	2005/01	Papers	Cytoprotection against oxidative stress-induced damage of astrocytes by extracellular ATP via P2Y1 receptors. Glia 49 (2),pp.288-300 (Co-authored)
68.	2004/12	Papers	Differential modulation of PI3-kinase/Akt pathway during all-trans retinoic acid- and Am80-induced HL-60 cell differentiation revealed by DNA microarray analysis. Biochemical pharmacology 68 (11),pp.2177-2186 (Co-authored)
69.	2004/04	Papers	Improvement in reliability of probabilistic test of significant differences in GeneChip experiments. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 20 (4),pp.731-733 (Co-authored)
70.	2004/03	Papers	Identification of novel alternative splice variants of human constitutive androstane receptor and characterization of their expression in the liver. Molecular pharmacology 65 (3),pp.496-502 (Co-authored)
71.	2003/11	Papers	Test of significant differences with a priori probability in microarray experiments. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 19 (11),pp.1529-1535 (Co-authored)
72.	2003/07	Papers	Distinct gene expression phenotypes of cells lacking Rb and Rb family members. Cancer research 63 (13),pp.3716-3723 (Co-authored)
73.	2003/06	Papers	Gene expression phenotypic models that predict the activity of oncogenic pathways. Nature genetics 34 (2),pp.226-230 (Co-authored)
74.	2003/03	Papers	Non-synonymous single nucleotide alterations in the microsomal epoxide hydrolase gene and their functional effects. Xenobiotica; the fate of foreign compounds in biological systems 33 (3),pp.277-287 (Co-authored)
75.	2003/01	Papers	Clinically potential subclasses of retinoid synergists revealed by gene expression profiling. Molecular cancer therapeutics 2 (1),pp.49-58 (Co-authored)
76.	2003/01	Papers	Glucuronidation of 7-ethyl-10-hydroxycamptothecin (SN-38), an active metabolite of irinotecan (CPT-11), by human UGT1A1 variants, G71R, P229Q, and Y486D. Drug metabolism and disposition: the biological fate of chemicals 31 (1),pp.108-113 (Co-authored)
77.	2002/12	Papers	Gene expression profiling of Ca2+-atpase inhibitor DTBHQ and antigen-stimulated RBL-2H3 mast cells. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 51 (12),pp.611-618 (Co-authored)
78.	2002/08	Papers	Characterization of human CYP1A1/1A2 induction by DNA microarray and alpha-naphthoflavone. Biochemical and biophysical research communications 296 (1),pp.172-177 (Co-authored)
79.	2002/07	Papers	Substitution of arginine for cysteine 643 of the glucocorticoid receptor reduces its steroid-binding affinity and transcriptional activity. Cancer letters 181 (1),pp.109-1014 (Co-authored)
80.	2002/04	Papers	Polymorphisms in the ABCC2 (cMOAT/MRP2) gene found in 72 established cell lines derived from Japanese individuals: an association between single nucleotide polymorphisms in the 5'-untranslated region and exon 28. Drug metabolism and disposition: the biological fate of chemicals 30 (4),pp.363-364 (Co-authored)
81.	2002	Papers	CYP3A4 gene polymorphisms influence testosterone 6beta-hydroxylation. Drug metabolism and pharmacokinetics 17 (2),pp.150-156 (Co-authored)
82.	2002	Papers	Determination of CYP2D6 gene alleles by the CYP450 probe array using the Affymetrix GeneChip system: comparison with sequencing results. Drug metabolism and pharmacokinetics 17 (2),pp.157-160 (Co-authored)
83.	2001/12	Papers	Non-synonymous single nucleotide alterations found in the CYP2C8 gene result in reduced in vitro paclitaxel metabolism. Biological & pharmaceutical bulletin 24 (12),pp.1427-1430 (Co-authored)
84.	2001/09	Papers	Predicting the clinical status of human breast cancer by using gene expression profiles. Proceedings of the National Academy of Sciences of the United States of America 98 (20),pp.11462-11467 (Co-authored)
85.	2001/07	Papers	Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis. Molecular and cellular biology 21 (14),pp.4684-4699 (Co-authored)
86.	2000/09	Papers	E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control. Molecular cell 6 (3),pp.729-735 (Co-authored)
87.	2000/08	Papers	Loss of E2F4 activity leads to abnormal development of multiple cellular lineages. Molecular cell 6 (2),pp.293-306 (Co-authored)
88.	2000/05	Papers	Identification of a novel E2F3 product suggests a mechanism for determining specificity of repression by Rb proteins. Molecular and cellular biology 20 (10),pp.3626-3632 (Co-authored)
89.	1998/07	Papers	E2F3 activity is regulated during the cell cycle and is required for the induction of S phase. Genes & development 12 (14),pp.2120-2130 (Co-authored)
90.	1997/06	Papers	Transcriptional activation of the human c-myc gene by simian virus 40 large T antigen without binding to p53 and RB proteins in the transient expression system. Biochemical and biophysical research communications 235 (1),pp.153-157 (Co-authored)
91.	1997/04	Papers	Isolation and analysis of cellular DNA fragments directly binding to c-Myc protein. Leukemia 11 Suppl 3,pp.399-401 (Co-authored)
92.	1995/06	Papers	Characterization of cis-regulatory elements of the c-myc promoter responding to human GM-CSF or mouse interleukin 3 in mouse proB cell line BA/F3 cells expressing the human GM-CSF receptor. Molecular biology of the cell 6 (6),pp.627-636 (Co-authored)
93.	1995/03	Papers	A direct role of transcription factor E2F in c-myc gene expression during granulocytic and macrophage-like differentiation of HL60 cells. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 6 (3),pp.229-237 (Co-authored)
94.	1994/03	Papers	Transcription from the P2 promoter of human protooncogene myc is suppressed by retinoic acid through an interaction between the E2F element and its binding proteins. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 5 (3),pp.287-294 (Co-authored)